Plasmid_Backbone

Part:BBa_K563050:Design

Designed by: Guo Jing   Group: iGEM11_Tianjin   (2011-10-05)


pYE+LacZ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 167
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 153
    Illegal XhoI site found at 133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 167
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1248


Design Notes

We use LacZ to report the work of gene circuit.


Source

LacZ is from S.cerevisivae. pYE is bought from invitrogen.

References